In our lab, the research on stroke is conducted in order to achieve a fundamental understanding of how oxygen deprivation affects the brain tissue and how it activates the defense mechanisms that can prevent the brain-damage. More knowledge will hopefully lead to establish new therapeutic and preventive strategies at the cellular and molecular level to those at risk for stroke.
The study is based on the discovery of a hemoprotein, Neuroglobin (Ngb), found in various parts of the brain, the pancreas and in the retina. Studies have shown that the protein has a protective functional role against hypoxia-related brain-damage. How this happens, is in the current situation, not clear.
In our group, techniques are developed and combined to explore the real-time response of functional single cells in different chemical environments. We aim to study and understand Ngbs effect on neurons signaling ability and electrophysiological activity under hypoxic (low oxygen) and anoxic (oxygen free) conditions using patch-clamp technique and optical spectroscopy.
Precise electrophysiological measurements
In order to perform precise electrophysiological measurements under well-controlled physiological conditions, patch clamp is included in a multi-functional system of a developed gas-tight microfluidic combined with optical manipulation technique and optical spectroscopy.
The setup is built on a vibration-free optical table (Technical Manufacturing Corporation, TMC, USA), where the the techniques are integrated on an inverted microscope (IX 71, Olympus, Japan).
The project's long-term goals
The long-term goal is to develop a multifunctional system to understand the behavior, response and defense mechanisms of the cell in oxygen- shortage conditions related to Ngb, which can create new ways to interpret other hypoxia-related diseases such as Alzheimer's and Parkinson's.
The project's long-term goal is to use this system to understand cell behavior, response and defense mechanisms in oxygen-poor conditions related to Ngb, which can create new ways to interpret other hypoxia-related diseases such as Alzheimer's and Parkinson's.