High Performance Liquid Chromatography, HPLC, is a common technique for separation, identification and quantification of various compounds. The sample is transported with a mobile phase to a column (stationary phase) where the separation takes place, since different compounds are retained to the column for different times. For detection of the compounds, some kind of optical detector is usually applied, such as UV, fluorescence or RI detector. By varying the column, mobile phase and detector, a wide range of separation systems can be designed.
AB Sciex API 3200™ LC/MS/MS System
Perkin Elmer series 200 pump
Perkin Elmer series 200 UV/VIS detektor
Perkin Elmer series 200 Refractive Index detector
Perkin Elmer series 200 Autosampler
Analytical and semi-preparative columns
The equipment is for example utilized for analysis of sugars and organic acids and for peptide purification.